This product is available in our Quick-Load Purple format - enjoy the convenience of a ready-to-load format.
*On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca
- Size range: 25 bp to 766 bp
- Value pricing
- Supplied with free vial of Gel Loading Dye, Purple (6X), no SDS (NEB #B7025).
- Easy to identify reference bands
- Small size suitable for 100 gel lanes; large size suitable for 500 gel lanes
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- Product Information
Comes supplied with 1 vial of Gel Loading Dye, Purple (6X), no SDS.
Reagents Supplied
The following reagents are supplied with this product:
| NEB # | Component Name | Component # | Stored at (°C) | Amount | Concentration |
| N3233S | -20 |
| Low Molecular Weight DNA Ladder | N3233SVIAL | -20 | 1 x 0.1 ml | 500 µg/ml | |
| Gel Loading Dye, Purple (6X), no SDS | B7025SVIAL | 25 | 1 x 1 ml | 6 X |
Properties & Usage
Bases
| Fragment | Mass (ng) | bp |
|---|---|---|
| 1 | 42 | 766 |
| 2 | 27 | 500 |
| 3 | 20 | 350 |
| 4 | 33 | 300 |
| 5 | 27 | 250 |
| 6 | 110 | 200 |
| 7 | 33 | 150 |
| 8 | 43 | 100 |
| 9 | 58 | 75 |
| 10 | 63 | 50 |
| 11 | 43 | 25 |
Effective Size Range
25bp to 766bp
Storage Buffer
10 mM Tris-HCl
1 mM EDTA
pH 8 @ 25°C
Companion Products
- Gel Loading Dye, Purple (6X), no SDS
- Monarch® Plasmid Miniprep Kit
- Monarch® DNA Gel Extraction Kit
Materials Sold Separately
- Gel Loading Dye, Purple (6X), no SDS
Notes
- We recommend loading 0.5 µg of the Low Molecular Weight DNA Ladder diluted in sample buffer.
- This ladder was not designed for precise quantification of DNA mass but can be used for approximating the mass of DNA in comparably intense samples of similar size.
- All ends have 5´ overhangs that can be end labeled using T4 Polynucleotide Kinase (#M0201) or filled-in using DNA Polymerase I, Klenow Fragment (#M0210) (1). Use α-[32P] dCTP or α-[32P] dGTP for the fill-in reaction.
- DNA ladders are stable for at least 3 months at 4°C.
- For long term storage, store at -20°C. If samples need to be diluted, use TE or other buffer of minimal ionic strength. DNA may denature if diluted in dH2O
- 1X Gel Loading Dye, Purple, no SDS:
2.5% Ficoll®-400
10mM EDTA
3.3mM Tris-HCl (pH 8.0@25°C)
0.02% Dye 1
0.001% Dye 2 - Due to the limitations of the acrylamide gel technology, one or two extra bands may be visible on the DNA ladders when run on a polyacrylamide gel.
References
- Sambrook, J., Fritsch, E.F. and Maniatis, T. (1989). Molecular Cloning: A Laboratory Manual, (2nd ed. 10.51-10.67.
Protocols
- End-labeling Protocol
Selection Charts
FAQs
- Why are there extra bands visible on polyacrylamide gels?
- Why is the separation of the lower bands incomplete?
- What are the overhangs on the DNA ladder fragments? Can I end-label them using the T4 polynucleotide kinase (PNK)? What about the Klenow fragment?
- Why are the DNA ladders showing up on my Southern blot? What is the sequence or composition of the ladder bands?
- How can I quantify the amount of DNA in each band of a marker?
- Can I use Midori Green with the DNA Ladders from NEB?
- Can I use SYBR®and/or GelRed®dyes with the DNA Ladders from NEB?
- Why is my DNA Ladder floating out the wells? Why are there no bands visible in my DNA ladder gel lane?
- Can I use GelRed®with the Low Molecular Weight and 50 bp DNA Ladders from NEB?
Tech Tips
- To make it ready-to-load, dilute in TE buffer instead of water.
Citations
Quality Control Assay
Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.
Specifications
The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]
Certificate of Analysis
The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]
- N3233S_L_v1_0091401
- N3233S_L_v1_0091407
- N3233S_L_v1_0091412
- N3233S_L_v1_0091505
- N3233S_L_v1_0101507
- N3233S_L_v1_0101511
- N3233S_L_v1_0101604
- N3233S_L_v1_0101608
- N3233S_L_v1_0101708
- N3233S_L_v1_0111804
- N3233S_v1_10022866
- N3233L_v1_10022864
- N3233S_v1_10042930
- N3233L_v1_10050601
- N3233L_v1_10055032
- N3233S_v1_10060890
- N3233S_v1_10068134
- N3233S_v1_10078356
- N3233S_v1_10086397
- N3233L_v1_10078784
- N3233L_v1_10089135
- N3233S_v1_10095182
- N3233L_v1_10106989
- N3233S_v1_10106992
- N3233L_v1_10122986
- N3233S_v1_10136149
- N3233L_v1_10149521
- N3233S_v1_10149523
- N3233S_v1_10176777
- N3233L_v1_10184491
- N3233S_v1_10213819
- N3233L_v1_10210482
- N3233L_v1_10242441
Safety Data Sheets
Legal And Disclaimer
Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.
This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.
